immunofluorescence staining for desmin  (Proteintech)

Journal: Regenerative Therapy

Article Title: miR-145-enriched BMSCs-derived exosomes ameliorate neurogenic erectile dysfunction in aged rats via TGFBR2 inhibition

doi: 10.1016/j.reth.2025.04.004

Figure Lengend Snippet: Characterization of CCSMCs and their interaction with exosomes . (A) Primary CCSMCs migrating from cavernous tissue explants. (B) Immunofluorescence staining with anti-α-SMA and anti-Desmin antibodies confirming the identity of CCSMCs. (C) Internalization of PKH67-labeled Exo and Exo-145 by CCSMCs, showing green fluorescence within the cytoplasm. (D) Elevated miR-145 expression levels in CCSMCs after co-culture with Exo or Exo-145. Data are presented as mean ± SEM. ∗∗∗ p < 0.001, ns: no significant difference.

Article Snippet: Third-generation CCSMCs were identified through immunofluorescence staining for desmin (1:200, 16520-1-AP, Proteintech) and α-smooth muscle actin (1:200, 14395-1-AP, Proteintech).

Techniques: Immunofluorescence, Staining, Labeling, Fluorescence, Expressing, Co-Culture Assay

Journal: Nanomedicine (London, England)

Article Title: Porous Se@SiO 2 nanoparticles improve oxidative injury to promote muscle regeneration via modulating mitochondria.

doi: 10.2217/nnm-2022-0173

Figure Lengend Snippet: Figure 1. Characterization of the solid or porous Se@SiO2 NPs and identification of satellite cells. (A) Fabrication process of porous Se@SiO2 NPs. (B) x-ray diffractometer pattern of the solid Se@SiO2 NPs and the typical selenium nanocrystals (Joint Committee on Powder Diffraction Standards card no. 65–1,876). (C) Low and (D) high magnification of transmission electron microscopy images of the solid Se@SiO2 NPs. (E) Transmission electron microscopy images of porous Se@SiO2 NPs. (F) PAX7 and desmin were detected by immunofluorescence; cytoskeletal alterations were detected by staining with F-actin. The nuclei of satellite cells were stained with Hoechst. The scale bar represents 20 μm. (G) Cell proliferation in satellite cells in response to different porous Se@SiO2 NP concentrations. *p < 0.05; **p < 0.01; ***p < 0.001 compared with control (0 μg/ml). DIW: Deionized water; NP: Nanoparticle; PVP: Polyvinylpyrrolidone; TEOS: Tetraethyl orthosilicate.

Article Snippet: Immunofluorescence staining With immunofluorescence staining, the expression of markers was detected using antibodies against desmin (Proteintech), PAX7 (Proteintech), MYHC (Thermo Fisher Scientific), MYOD (Invitrogen), F-actin (Abcam) and dystrophin (Santa Cruz Biotechnology, TX, USA).

Techniques: Transmission Assay, Electron Microscopy, Immunofluorescence, Staining, Control

Journal: Nanomedicine (London, England)

Article Title: Porous Se@SiO 2 nanoparticles improve oxidative injury to promote muscle regeneration via modulating mitochondria.

doi: 10.2217/nnm-2022-0173

Figure Lengend Snippet: Figure 7. Porous Se@SiO2 nanoparticles promote the formation of myotubes and increase the number of PAX7-positive cells. (A) Western blot analysis of MYHC, MYOD and GAPDH expression of satellite cells in each group after normalization to GAPDH expression; the comparison is shown in (B) and (C). (D) Immunofluorescence staining of MYHC was observed with dystrophin staining as background. Immunofluorescence analysis for MYHC (red) and dystrophin (green). Nuclei were stained with Hoechst, displayed in blue. The scale bar represents 20 μm. (E) Quantification of MYHC mean fluorescence intensity. (F) Representative images of PAX7 observed with dystrophin staining as background. Immunofluorescence analysis of PAX7 (red) and dystrophin (green). Nuclei were stained with Hoechst, displayed in blue. The scale bar represents 20 μm. (G) Quantification of PAX7 expression. *p < 0.05; **p < 0.01; ***p < 0.001. CTX: Cardiotoxin.

Article Snippet: Immunofluorescence staining With immunofluorescence staining, the expression of markers was detected using antibodies against desmin (Proteintech), PAX7 (Proteintech), MYHC (Thermo Fisher Scientific), MYOD (Invitrogen), F-actin (Abcam) and dystrophin (Santa Cruz Biotechnology, TX, USA).

Techniques: Western Blot, Expressing, Comparison, Immunofluorescence, Staining, Fluorescence

Journal: Bioengineered

Article Title: Echinococcus multilocularis drives the polarization of macrophages by regulating the RhoA-MAPK signaling pathway and thus affects liver fibrosis

doi: 10.1080/21655979.2022.2056690

Figure Lengend Snippet: The effect of macrophage transformation on the activation of hepatic stellate cells by immunofluorescence staining of α-SMA. * P < 0.05 vs. AEm group.

Article Snippet: Anti-desmin antibody (5332, 1:100) for immunofluorescence staining was obtained from Cell Signaling.

Techniques: Transformation Assay, Activation Assay, Immunofluorescence, Staining